A novel chromatographic separation system and ion exchange resins

The chromatographic separation and purification of acidic proteins on carboxylated ion exchange resins.

The chromatographic separation of proteins on carboxylated ion exchange resins has been widely employed (1, 2). However, as pointed out previously by several authors (l-6), only proteins with alkaline or neutral isoelectric points have been investigated. This report deals with a systematic study of the behavior of proteins with acid isoelectric points on weak cation exchange resins.l In view of...

Ion-exchange chromatographic separation and fluorometric detection of urinary polyamines.

An ion-exchange chromatographic procedure has been developed for the separation and fluorometric detection of the urinary polyamines 1,3-diaminopropane, putrescine, spermidine, cadaverine, and spermine. The polyamines were separated on a 15 x0.45 cm cation-exchange column (70”) employing a combined pH-salt gradient. The separation conditions were designed to enable utilization of the sensitive ...

[Ion exchange resins].

Ion exchange resins have been used to help formulate pharmaceuticals since the late 1950's. During that time they have proved to be safe and effective excipients and are now used in many commercial formulations throughout the world. In this article we will look at some of the common problems faced by formulators and how using ion exchange resins may be able to solve them. Ion exchange resins ar...

Displacement chromatography on synthetic ion-exchange resins. 1. Separation of organic bases and amino-acids using cation-exchange resins.

9. Accumulation of glutamate also occurred in isolated kidney cortex, spleen, lung and chorion. Six other tissues examined maintained a concentration gradient between tissue and medium, but did not increase the glutamate concentration above the original level. 10. It is pointed out that tissue slices afford a system in which problems of permeability and storage can be studied under controlled c...

Ion exchange resins: catalyst recovery and recycle.